Journal: Frontiers in Cellular and Infection Microbiology
Article Title: CD8 + T cells promote tubule-interstitial damage in malaria-induced acute kidney injury
doi: 10.3389/fcimb.2025.1561806
Figure Lengend Snippet: Adoptive transfer of malaria-responsive T cells induces an increase in CD8 + T cells population in renal tissue. (A) Dot-plot representing the percentage of CD4 and CD8 + T cells in total CD45 + renal cells of the naive → naive (orange) and infected → naive (blue) groups. (B) Quantification of the percentage of CD4 + (n = 5) and (C) CD8 + T cells (n = 5) in the kidney. (D) Representative immunoblotting of cortical perforin-1 expression in renal tissue. (E) Quantification of perforin-1 expression in renal tissue (n = 4). *versus naive → naive group, P < 0.05.
Article Snippet: The cells were incubated with the following antibodies for surface markers: PeCy5.5-conjugated rat IgG2a anti-murine CD8 (45-0081-80, Thermo Fisher Scientific, Waltham, MA), PE-conjugated rat IgG2b anti-mouse CD4 (clone GK1.5; 11-0041-82, Thermo Fisher Scientific), and FITC-conjugated rat IgG1 anti-mouse CD45 (11.0452-82, Thermo Fisher Scientific).
Techniques: Adoptive Transfer Assay, Infection, Western Blot, Expressing